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A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer

High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cryopreservation, these methods have been limited to...

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Detalles Bibliográficos
Autores principales: Ebanks, Brad, Kwiecinska, Pola, Moisoi, Nicoleta, Chakrabarti, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365301/
https://www.ncbi.nlm.nih.gov/pubmed/37486925
http://dx.doi.org/10.1371/journal.pone.0276147