Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations

Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes. They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As such, they hold great potential for the engineering of microbes...

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Detalles Bibliográficos
Autores principales: Seys, François M., Humphreys, Christopher M., Tomi-Andrino, Claudio, Li, Qi, Millat, Thomas, Yang, Sheng, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366002/
https://www.ncbi.nlm.nih.gov/pubmed/37496853
http://dx.doi.org/10.3389/fbioe.2023.1211197

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366002/
https://www.ncbi.nlm.nih.gov/pubmed/37496853
http://dx.doi.org/10.3389/fbioe.2023.1211197

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