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Isoform-specific, Semi-quantitative Determination of Highly Homologous Protein Levels via CRISPR-Cas9-mediated HiBiT Tagging
Many protein families consist of multiple highly homologous proteins, whether they are encoded by different genes or originating from the same genomic location. Predominance of certain isoforms has been linked to various pathological conditions, such as cancer. Detection and relative quantification...
Autores principales: | Seiler, Kristina, Rafiq, Sreoshee, Tschan, Mario P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366989/ https://www.ncbi.nlm.nih.gov/pubmed/37497448 http://dx.doi.org/10.21769/BioProtoc.4777 |
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