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Production and Purification of Cell Culture–generated Hepatitis B Virus by Transient Transfection and Density Gradient

An efficient cell culture system for hepatitis B virus (HBV) is indispensable for research on viral characteristics and antiviral agents. Currently, for HBV infection assays in cell culture, HBV genome-integrated cell line–derived viruses are commonly used. However, these viruses are not suitable fo...

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Detalles Bibliográficos
Autores principales: Murayama, Asako, Akari, Hirofumi, Kato, Takanobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366991/
https://www.ncbi.nlm.nih.gov/pubmed/37497458
http://dx.doi.org/10.21769/BioProtoc.4779