Cargando…
Production and Purification of Cell Culture–generated Hepatitis B Virus by Transient Transfection and Density Gradient
An efficient cell culture system for hepatitis B virus (HBV) is indispensable for research on viral characteristics and antiviral agents. Currently, for HBV infection assays in cell culture, HBV genome-integrated cell line–derived viruses are commonly used. However, these viruses are not suitable fo...
Autores principales: | Murayama, Asako, Akari, Hirofumi, Kato, Takanobu |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366991/ https://www.ncbi.nlm.nih.gov/pubmed/37497458 http://dx.doi.org/10.21769/BioProtoc.4779 |
Ejemplares similares
-
Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
por: Murayama, Asako, et al.
Publicado: (2016) -
Purification and production of Plasmodium falciparum zygotes from in vitro culture using magnetic column and Percoll density gradient
por: Zhou, Yaxian, et al.
Publicado: (2020) -
Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells
por: Cupo, Albert, et al.
Publicado: (2019) -
A solid‐phase transfection platform for arrayed CRISPR screens
por: Serçin, Özdemirhan, et al.
Publicado: (2019) -
Single Strain Isolation Method for Cell Culture-Adapted Hepatitis C Virus by End-Point Dilution and Infection
por: Sugiyama, Nao, et al.
Publicado: (2014)