Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET

Correlative cryo-FLM-FIB milling is a powerful sample preparation technique for in situ cryo-ET. However, correlative workflows that incorporate precise targeting remain challenging. Here, we demonstrate the development and use of an integrated Fluorescence Light Microscope (iFLM) module within a cr...

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Detalles Bibliográficos
Autores principales: Yang, Jae, Vrbovská, Veronika, Franke, Tilman, Sibert, Bryan, Larson, Matthew, Hall, Alex, Rigort, Alex, Mitchels, John, Wright, Elizabeth R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369943/
https://www.ncbi.nlm.nih.gov/pubmed/37502891
http://dx.doi.org/10.1101/2023.07.11.548578

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369943/
https://www.ncbi.nlm.nih.gov/pubmed/37502891
http://dx.doi.org/10.1101/2023.07.11.548578

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