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Determining growth rates from bright-field images of budding cells through identifying overlaps

Much of biochemical regulation ultimately controls growth rate, particularly in microbes. Although time-lapse microscopy visualises cells, determining their growth rates is challenging, particularly for those that divide asymmetrically, like Saccharomyces cerevisiae, because cells often overlap in i...

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Detalles Bibliográficos
Autores principales: Pietsch, Julian MJ, Muñoz, Alán F, Adjavon, Diane-Yayra A, Farquhar, Iseabail, Clark, Ivan BN, Swain, Peter S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371227/
https://www.ncbi.nlm.nih.gov/pubmed/37417869
http://dx.doi.org/10.7554/eLife.79812