Protocol for siRNA-mediated TET1 knockdown during differentiation of human embryonic stem cells into definitive endoderm cells

TET1-mediated active DNA demethylation is required for endogenous retrovirus (ERV) enhancer activation during human ES differentiation into definitive endoderm (DE) cells. Here we present a protocol for siRNA-mediated TET1 knockdown during this process to decipher TET1’s role in ERV activation and D...

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Detalles Bibliográficos
Autores principales: Liufu, Zhongqi, Liu, Yujian, He, Jiangping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371804/
https://www.ncbi.nlm.nih.gov/pubmed/37467109
http://dx.doi.org/10.1016/j.xpro.2023.102455

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371804/
https://www.ncbi.nlm.nih.gov/pubmed/37467109
http://dx.doi.org/10.1016/j.xpro.2023.102455

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