Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation

[Image: see text] An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradi...

Descripción completa

Detalles Bibliográficos
Autores principales: Janosko, Chasity P., Shade, Olivia, Courtney, Taylor M., Horst, Trevor J., Liu, Melinda, Khare, Sagar D., Deiters, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373180/
https://www.ncbi.nlm.nih.gov/pubmed/37521667
http://dx.doi.org/10.1021/acsomega.3c03512
_version_ 1785078509626982400
author Janosko, Chasity P.
Shade, Olivia
Courtney, Taylor M.
Horst, Trevor J.
Liu, Melinda
Khare, Sagar D.
Deiters, Alexander
author_facet Janosko, Chasity P.
Shade, Olivia
Courtney, Taylor M.
Horst, Trevor J.
Liu, Melinda
Khare, Sagar D.
Deiters, Alexander
author_sort Janosko, Chasity P.
collection PubMed
description [Image: see text] An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans-configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.
format Online
Article
Text
id pubmed-10373180
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-103731802023-07-28 Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation Janosko, Chasity P. Shade, Olivia Courtney, Taylor M. Horst, Trevor J. Liu, Melinda Khare, Sagar D. Deiters, Alexander ACS Omega [Image: see text] An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the trans-configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure. American Chemical Society 2023-07-14 /pmc/articles/PMC10373180/ /pubmed/37521667 http://dx.doi.org/10.1021/acsomega.3c03512 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Janosko, Chasity P.
Shade, Olivia
Courtney, Taylor M.
Horst, Trevor J.
Liu, Melinda
Khare, Sagar D.
Deiters, Alexander
Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
title Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
title_full Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
title_fullStr Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
title_full_unstemmed Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
title_short Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation
title_sort genetic encoding of arylazopyrazole phenylalanine for optical control of translation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373180/
https://www.ncbi.nlm.nih.gov/pubmed/37521667
http://dx.doi.org/10.1021/acsomega.3c03512
work_keys_str_mv AT janoskochasityp geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation
AT shadeolivia geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation
AT courtneytaylorm geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation
AT horsttrevorj geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation
AT liumelinda geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation
AT kharesagard geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation
AT deitersalexander geneticencodingofarylazopyrazolephenylalanineforopticalcontroloftranslation

Ejemplares similares