Cargando…

Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits

Dynamic, multi-input gene regulatory networks (GRNs) are ubiquitous in nature. Multilayer CRISPR-based genetic circuits hold great promise for building GRNs akin to those found in naturally occurring biological systems. We develop an approach for creating high-performing activatable promoters that c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alba Burbano, Diego, Cardiff, Ryan A. L., Tickman, Benjamin I., Kiattisewee, Cholpisit, Maranas, Cassandra J., Zalatan, Jesse G., Carothers, James M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	National Academy of Sciences 2023
Materias:	Biological Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374173/ https://www.ncbi.nlm.nih.gov/pubmed/37463216 http://dx.doi.org/10.1073/pnas.2220358120

Ejemplares similares

Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
por: Fontana, Jason, et al.
Publicado: (2020)

Gene expression dynamics in input-responsive engineered living materials programmed for bioproduction
por: Sugianto, Widianti, et al.
Publicado: (2023)

Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria
por: Liu, Yang, et al.
Publicado: (2019)

Promoting CRISPRa for Targeted Treatment of Epilepsy
por: Wagnon, Jacy L.
Publicado: (2020)

Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria
por: Dong, Chen, et al.
Publicado: (2018)

Author Correction: Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria
por: Dong, Chen, et al.
Publicado: (2018)

Precise tumor immune rewiring via synthetic CRISPRa circuits gated by concurrent gain/loss of transcription factors
por: Wang, Yafeng, et al.
Publicado: (2022)

Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa
por: Lau, Cia-Hin, et al.
Publicado: (2019)

Porcine antiviral activity is increased by CRISPRa-SAM system
por: Jiang, Jinhe, et al.
Publicado: (2019)

Off-target effects of CRISPRa on interleukin-6 expression
por: Soubeyrand, Sébastien, et al.
Publicado: (2019)

Rationally-engineered reproductive barriers using CRISPR & CRISPRa: an evaluation of the synthetic species concept in Drosophila melanogaster
por: Waters, Andrew J., et al.
Publicado: (2018)

CRISPRa-mediated FOXP3 gene upregulation in mammalian cells
por: Forstnerič, Vida, et al.
Publicado: (2019)

Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi
por: Jensen, Trine I., et al.
Publicado: (2021)

Multiplexed activation of endogenous genes by CRISPRa elicits potent anti-tumor immunity
por: Wang, Guangchuan, et al.
Publicado: (2019)

Scalable input/output: achieving system balance
por: Reed, Daniel A, et al.
Publicado: (2003)

Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA
por: Lundh, Morten, et al.
Publicado: (2017)

Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming
por: Yang, Jian, et al.
Publicado: (2019)

Targeted Modulation of Chicken Genes In Vitro Using CRISPRa and CRISPRi Toolkit
por: Chapman, Brittany, et al.
Publicado: (2023)

OptCircuit: An optimization based method for computational design of genetic circuits
por: Dasika, Madhukar S, et al.
Publicado: (2008)

Tumour suppression by targeted intravenous non-viral CRISPRa using dendritic polymers
por: Kretzmann, Jessica A., et al.
Publicado: (2019)

Maybe you can turn me on: CRISPRa-based strategies for therapeutic applications
por: Becirovic, Elvir
Publicado: (2022)

Parkinson's disease motor symptoms rescue by CRISPRa‐reprogramming astrocytes into GABAergic neurons
por: Giehrl‐Schwab, Jessica, et al.
Publicado: (2022)

Different transcriptional responses by the CRISPRa system in distinct types of heterochromatin in Drosophila melanogaster
por: Ortega-Yáñez, Andrea, et al.
Publicado: (2022)

Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice
por: Hunt, Charleen, et al.
Publicado: (2021)

In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy
por: Colasante, Gaia, et al.
Publicado: (2020)

In silico performance analysis of web tools for CRISPRa sgRNA design in human genes
por: Nuñez Pedrozo, Cristian N., et al.
Publicado: (2022)

Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma
por: Sgro, Agustin, et al.
Publicado: (2023)

Phase-separated DropCRISPRa platform for efficient gene activation in mammalian cells and mice
por: Ma, Shufeng, et al.
Publicado: (2023)

Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus
por: Kostyushev, Dmitry, et al.
Publicado: (2023)

Transplantation of CRISPRa system engineered IL10-overexpressing bone marrow-derived mesenchymal stem cells for the treatment of myocardial infarction in diabetic mice
por: Meng, Xin, et al.
Publicado: (2019)

Neural Circuits for Social Interactions: From Microcircuits to Input-Output Circuits
por: Xu, Sen, et al.
Publicado: (2021)

Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases
por: Brezgin, Sergey, et al.
Publicado: (2020)

Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants
por: Ding, Xiao, et al.
Publicado: (2022)

Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing Conditions
por: Arango, David, et al.
Publicado: (2023)

CRISPRa‐based activation of Fgf21 and Fndc5 ameliorates obesity by promoting adipocytes browning
por: Zhu, Hongtao, et al.
Publicado: (2023)

Acoustomechanically activatable liposomes for ultrasonic drug uncaging
por: Purohit, Mahaveer P., et al.
Publicado: (2023)

Input clustering and the microscale structure of local circuits
por: DeBello, William M., et al.
Publicado: (2014)

Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit
por: de Melo, Bruno Paes, et al.
Publicado: (2020)

Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform
por: Siepe, Dirk H, et al.
Publicado: (2022)

DIPG-03. IDENTIFICATION OF NEW THERAPEUTIC TARGETS BY ARRAYED CRISPRA-BASED APPROACH TO MAP RADIORESISTANCE IN DMG
por: Fernandez, Solène, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_m3ikgeln2nrdl5les51nsl789k