FISH-Flow to quantify nascent and mature ribosomal RNA in mouse and human cells

FISH-Flow (fluorescence in situ hybridization-flow cytometry) involves hybridizing fluorescent oligos to RNA and quantifying fluorescence at a single-cell level using flow cytometry. Here, we present a FISH-Flow protocol to quantify nascent 47S and mature 18S and 28S rRNAs in mouse and human cells,...

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Detalles Bibliográficos
Autores principales: Antony, Charles, Somers, Patrick, Gray, Erin M., Pimkin, Maxim, Paralkar, Vikram R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374870/
https://www.ncbi.nlm.nih.gov/pubmed/37481729
http://dx.doi.org/10.1016/j.xpro.2023.102463

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374870/
https://www.ncbi.nlm.nih.gov/pubmed/37481729
http://dx.doi.org/10.1016/j.xpro.2023.102463

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