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Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity

This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR) polymer. The detection system was designed b...

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Detalles Bibliográficos
Autores principales: Gao, Fengli, Liu, Gang, Qiao, Yishu, Dong, Xiuwen, Liu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377026/
https://www.ncbi.nlm.nih.gov/pubmed/37504121
http://dx.doi.org/10.3390/bios13070723