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Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging

In this work, we obtained three new phosphorescent iridium complexes (Ir1–Ir3) of general stoichiometry [Ir(N^C)(2)(N^N)]Cl decorated with oligo(ethylene glycol) fragments to make them water-soluble and biocompatible, as well as to protect them from aggregation with biomolecules such as albumin. The...

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Autores principales: Samandarsangari, Mozhgan, Kozina, Daria O., Sokolov, Victor V., Komarova, Anastasia D., Shirmanova, Marina V., Kritchenkov, Ilya S., Tunik, Sergey P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377268/
https://www.ncbi.nlm.nih.gov/pubmed/37504079
http://dx.doi.org/10.3390/bios13070680
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author Samandarsangari, Mozhgan
Kozina, Daria O.
Sokolov, Victor V.
Komarova, Anastasia D.
Shirmanova, Marina V.
Kritchenkov, Ilya S.
Tunik, Sergey P.
author_facet Samandarsangari, Mozhgan
Kozina, Daria O.
Sokolov, Victor V.
Komarova, Anastasia D.
Shirmanova, Marina V.
Kritchenkov, Ilya S.
Tunik, Sergey P.
author_sort Samandarsangari, Mozhgan
collection PubMed
description In this work, we obtained three new phosphorescent iridium complexes (Ir1–Ir3) of general stoichiometry [Ir(N^C)(2)(N^N)]Cl decorated with oligo(ethylene glycol) fragments to make them water-soluble and biocompatible, as well as to protect them from aggregation with biomolecules such as albumin. The major photophysical characteristics of these phosphorescent complexes are determined by the nature of two cyclometallating ligands (N^C) based on 2-pyridine-benzothiophene, since quantum chemical calculations revealed that the electronic transitions responsible for the excitation and emission are localized mainly at these fragments. However, the use of various diimine ligands (N^N) proved to affect the quantum yield of phosphorescence and allowed for changing the complexes’ sensitivity to oxygen, due to the variations in the steric accessibility of the chromophore center for O(2) molecules. It was also found that the N^N ligands made it possible to tune the biocompatibility of the resulting compounds. The wavelengths of the Ir1–Ir3 emission maxima fell in the range of 630–650 nm, the quantum yields reached 17% (Ir1) in a deaerated solution, and sensitivity to molecular oxygen, estimated as the ratio of emission lifetime in deaerated and aerated water solutions, displayed the highest value, 8.2, for Ir1. The obtained complexes featured low toxicity, good water solubility and the absence of a significant effect of biological environment components on the parameters of their emission. Of the studied compounds, Ir1 and Ir2 were chosen for in vitro and in vivo biological experiments to estimate oxygen concentration in cell lines and tumors. These sensors have demonstrated their effectiveness for mapping the distribution of oxygen and for monitoring hypoxia in the biological objects studied.
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spelling pubmed-103772682023-07-29 Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging Samandarsangari, Mozhgan Kozina, Daria O. Sokolov, Victor V. Komarova, Anastasia D. Shirmanova, Marina V. Kritchenkov, Ilya S. Tunik, Sergey P. Biosensors (Basel) Article In this work, we obtained three new phosphorescent iridium complexes (Ir1–Ir3) of general stoichiometry [Ir(N^C)(2)(N^N)]Cl decorated with oligo(ethylene glycol) fragments to make them water-soluble and biocompatible, as well as to protect them from aggregation with biomolecules such as albumin. The major photophysical characteristics of these phosphorescent complexes are determined by the nature of two cyclometallating ligands (N^C) based on 2-pyridine-benzothiophene, since quantum chemical calculations revealed that the electronic transitions responsible for the excitation and emission are localized mainly at these fragments. However, the use of various diimine ligands (N^N) proved to affect the quantum yield of phosphorescence and allowed for changing the complexes’ sensitivity to oxygen, due to the variations in the steric accessibility of the chromophore center for O(2) molecules. It was also found that the N^N ligands made it possible to tune the biocompatibility of the resulting compounds. The wavelengths of the Ir1–Ir3 emission maxima fell in the range of 630–650 nm, the quantum yields reached 17% (Ir1) in a deaerated solution, and sensitivity to molecular oxygen, estimated as the ratio of emission lifetime in deaerated and aerated water solutions, displayed the highest value, 8.2, for Ir1. The obtained complexes featured low toxicity, good water solubility and the absence of a significant effect of biological environment components on the parameters of their emission. Of the studied compounds, Ir1 and Ir2 were chosen for in vitro and in vivo biological experiments to estimate oxygen concentration in cell lines and tumors. These sensors have demonstrated their effectiveness for mapping the distribution of oxygen and for monitoring hypoxia in the biological objects studied. MDPI 2023-06-26 /pmc/articles/PMC10377268/ /pubmed/37504079 http://dx.doi.org/10.3390/bios13070680 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Samandarsangari, Mozhgan
Kozina, Daria O.
Sokolov, Victor V.
Komarova, Anastasia D.
Shirmanova, Marina V.
Kritchenkov, Ilya S.
Tunik, Sergey P.
Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging
title Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging
title_full Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging
title_fullStr Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging
title_full_unstemmed Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging
title_short Biocompatible Phosphorescent O(2) Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging
title_sort biocompatible phosphorescent o(2) sensors based on ir(iii) complexes for in vivo hypoxia imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377268/
https://www.ncbi.nlm.nih.gov/pubmed/37504079
http://dx.doi.org/10.3390/bios13070680
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