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Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly

Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequence...

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Detalles Bibliográficos
Autores principales: Xiong, Xingchen, Lu, Zhiwen, Ma, Lixin, Zhai, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377497/
https://www.ncbi.nlm.nih.gov/pubmed/37509059
http://dx.doi.org/10.3390/biom13071022