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Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly

Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequence...

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Autores principales: Xiong, Xingchen, Lu, Zhiwen, Ma, Lixin, Zhai, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377497/
https://www.ncbi.nlm.nih.gov/pubmed/37509059
http://dx.doi.org/10.3390/biom13071022
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author Xiong, Xingchen
Lu, Zhiwen
Ma, Lixin
Zhai, Chao
author_facet Xiong, Xingchen
Lu, Zhiwen
Ma, Lixin
Zhai, Chao
author_sort Xiong, Xingchen
collection PubMed
description Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequences at virtually any arbitrary site. The present review focuses on the commonly used in vivo and in vitro recombination-based gene cloning methods and the application of programmable endonucleases in these sequence- and ligation-independent DNA assembly methods. The advantages and shortcomings of the programmable endonucleases utilized as tools for gene cloning are also discussed in this review.
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spelling pubmed-103774972023-07-29 Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly Xiong, Xingchen Lu, Zhiwen Ma, Lixin Zhai, Chao Biomolecules Review Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequences at virtually any arbitrary site. The present review focuses on the commonly used in vivo and in vitro recombination-based gene cloning methods and the application of programmable endonucleases in these sequence- and ligation-independent DNA assembly methods. The advantages and shortcomings of the programmable endonucleases utilized as tools for gene cloning are also discussed in this review. MDPI 2023-06-21 /pmc/articles/PMC10377497/ /pubmed/37509059 http://dx.doi.org/10.3390/biom13071022 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Xiong, Xingchen
Lu, Zhiwen
Ma, Lixin
Zhai, Chao
Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
title Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
title_full Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
title_fullStr Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
title_full_unstemmed Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
title_short Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
title_sort applications of programmable endonucleases in sequence- and ligation-independent seamless dna assembly
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377497/
https://www.ncbi.nlm.nih.gov/pubmed/37509059
http://dx.doi.org/10.3390/biom13071022
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