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Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequence...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377497/ https://www.ncbi.nlm.nih.gov/pubmed/37509059 http://dx.doi.org/10.3390/biom13071022 |
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author | Xiong, Xingchen Lu, Zhiwen Ma, Lixin Zhai, Chao |
author_facet | Xiong, Xingchen Lu, Zhiwen Ma, Lixin Zhai, Chao |
author_sort | Xiong, Xingchen |
collection | PubMed |
description | Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequences at virtually any arbitrary site. The present review focuses on the commonly used in vivo and in vitro recombination-based gene cloning methods and the application of programmable endonucleases in these sequence- and ligation-independent DNA assembly methods. The advantages and shortcomings of the programmable endonucleases utilized as tools for gene cloning are also discussed in this review. |
format | Online Article Text |
id | pubmed-10377497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103774972023-07-29 Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly Xiong, Xingchen Lu, Zhiwen Ma, Lixin Zhai, Chao Biomolecules Review Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequences at virtually any arbitrary site. The present review focuses on the commonly used in vivo and in vitro recombination-based gene cloning methods and the application of programmable endonucleases in these sequence- and ligation-independent DNA assembly methods. The advantages and shortcomings of the programmable endonucleases utilized as tools for gene cloning are also discussed in this review. MDPI 2023-06-21 /pmc/articles/PMC10377497/ /pubmed/37509059 http://dx.doi.org/10.3390/biom13071022 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Xiong, Xingchen Lu, Zhiwen Ma, Lixin Zhai, Chao Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly |
title | Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly |
title_full | Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly |
title_fullStr | Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly |
title_full_unstemmed | Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly |
title_short | Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly |
title_sort | applications of programmable endonucleases in sequence- and ligation-independent seamless dna assembly |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377497/ https://www.ncbi.nlm.nih.gov/pubmed/37509059 http://dx.doi.org/10.3390/biom13071022 |
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