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Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly
Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequence...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377497/ https://www.ncbi.nlm.nih.gov/pubmed/37509059 http://dx.doi.org/10.3390/biom13071022 |