Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase

The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur. It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs). Impr...

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Detalles Bibliográficos
Autores principales: Wang, Jia-Hao, Wu, Su-Jun, Li, Yan, Zhao, Yue, Liu, Zhi-Mei, Deng, Shou-Long, Lian, Zheng-Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378061/
https://www.ncbi.nlm.nih.gov/pubmed/37508483
http://dx.doi.org/10.3390/cells12141818

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378061/
https://www.ncbi.nlm.nih.gov/pubmed/37508483
http://dx.doi.org/10.3390/cells12141818

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