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Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase
The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur. It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs). Impr...
Autores principales: | Wang, Jia-Hao, Wu, Su-Jun, Li, Yan, Zhao, Yue, Liu, Zhi-Mei, Deng, Shou-Long, Lian, Zheng-Xing |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378061/ https://www.ncbi.nlm.nih.gov/pubmed/37508483 http://dx.doi.org/10.3390/cells12141818 |
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