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Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector

The cashew peduncle has a high nutritional value and contains a wide variety of phenolic compounds. Among these, anacardic acids (AnAc) are biologically active components; however, they influence the cashew juice flavor and, consequently, its acceptance. This study validates a high-performance liqui...

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Autores principales: Oiram Filho, Francisco, Mitri, Morgana Pereira, Zocolo, Guilherme Julião, Canuto, Kirley Marques, de Brito, Edy Sousa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379927/
https://www.ncbi.nlm.nih.gov/pubmed/37509851
http://dx.doi.org/10.3390/foods12142759
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author Oiram Filho, Francisco
Mitri, Morgana Pereira
Zocolo, Guilherme Julião
Canuto, Kirley Marques
de Brito, Edy Sousa
author_facet Oiram Filho, Francisco
Mitri, Morgana Pereira
Zocolo, Guilherme Julião
Canuto, Kirley Marques
de Brito, Edy Sousa
author_sort Oiram Filho, Francisco
collection PubMed
description The cashew peduncle has a high nutritional value and contains a wide variety of phenolic compounds. Among these, anacardic acids (AnAc) are biologically active components; however, they influence the cashew juice flavor and, consequently, its acceptance. This study validates a high-performance liquid chromatography method for quantifying the AnAc present in cashew peduncles, using a C(18) reverse-phase column and a diode-array detector. The calibration curve obtained showed satisfactory precision for intraday (CV = 0.20%) and interday (CV = 0.29%) quantification, linearity (y = 2333.5x + 2956.2; r(2) = 0.9979), repeatability with respect to retention time (CV = 0.45%) and area (CV = 0.30%), and selectivity, and possessed detection and quantification limits of 0.18 and 0.85 µg·mL(−1), respectively. Different cashew clones containing AnAc were extracted and analyzed using the proposed method. A recovery of >90% was achieved using two sequential extractions. The total AnAc content ranged from 128.35 to 217.00 mg·100 g(−1) in peduncle samples obtained from five different cashew clones.
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spelling pubmed-103799272023-07-29 Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector Oiram Filho, Francisco Mitri, Morgana Pereira Zocolo, Guilherme Julião Canuto, Kirley Marques de Brito, Edy Sousa Foods Article The cashew peduncle has a high nutritional value and contains a wide variety of phenolic compounds. Among these, anacardic acids (AnAc) are biologically active components; however, they influence the cashew juice flavor and, consequently, its acceptance. This study validates a high-performance liquid chromatography method for quantifying the AnAc present in cashew peduncles, using a C(18) reverse-phase column and a diode-array detector. The calibration curve obtained showed satisfactory precision for intraday (CV = 0.20%) and interday (CV = 0.29%) quantification, linearity (y = 2333.5x + 2956.2; r(2) = 0.9979), repeatability with respect to retention time (CV = 0.45%) and area (CV = 0.30%), and selectivity, and possessed detection and quantification limits of 0.18 and 0.85 µg·mL(−1), respectively. Different cashew clones containing AnAc were extracted and analyzed using the proposed method. A recovery of >90% was achieved using two sequential extractions. The total AnAc content ranged from 128.35 to 217.00 mg·100 g(−1) in peduncle samples obtained from five different cashew clones. MDPI 2023-07-20 /pmc/articles/PMC10379927/ /pubmed/37509851 http://dx.doi.org/10.3390/foods12142759 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oiram Filho, Francisco
Mitri, Morgana Pereira
Zocolo, Guilherme Julião
Canuto, Kirley Marques
de Brito, Edy Sousa
Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
title Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
title_full Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
title_fullStr Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
title_full_unstemmed Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
title_short Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
title_sort validation of a method for anacardic acid quantification in cashew peduncles via high-performance liquid chromatography coupled to a diode-array detector
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379927/
https://www.ncbi.nlm.nih.gov/pubmed/37509851
http://dx.doi.org/10.3390/foods12142759
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