Photo-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and Prototrophic E. coli in Minimal Medium

Residue-specific incorporation of non-canonical amino acids (ncAAs) introduces bio-orthogonal functionalities into proteins. As such, this technique is applied in protein characterization and quantification. Here, we studied protein expression with three methionine analogs, namely photo-methionine (...

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Detalles Bibliográficos
Autores principales: Jecmen, Tomas, Tuzhilkin, Roman, Sulc, Miroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380393/
https://www.ncbi.nlm.nih.gov/pubmed/37511538
http://dx.doi.org/10.3390/ijms241411779

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380393/
https://www.ncbi.nlm.nih.gov/pubmed/37511538
http://dx.doi.org/10.3390/ijms241411779

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