Cargando…

Low-Density Lipoprotein Receptor (LDLR) Is Involved in Internalization of Lentiviral Particles Pseudotyped with SARS-CoV-2 Spike Protein in Ocular Cells

Here, we present evidence that caveolae-mediated endocytosis using LDLR is the pathway for SARS-CoV-2 virus internalization in the ocular cell line ARPE-19. Firstly, we found that, while Angiotensin-converting enzyme 2 (ACE2) is expressed in these cells, blocking ACE2 by antibody treatment did not p...

Descripción completa

Detalles Bibliográficos
Autores principales:	Uppal, Sheetal, Postnikova, Olga, Villasmil, Rafael, Rogozin, Igor B., Bocharov, Alexander V., Eggerman, Thomas L., Poliakov, Eugenia, Redmond, T. Michael
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380832/ https://www.ncbi.nlm.nih.gov/pubmed/37511618 http://dx.doi.org/10.3390/ijms241411860

Ejemplares similares

The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA
por: Postnikova, Olga A., et al.
Publicado: (2021)

Palmitoylation of Metazoan Carotenoid Oxygenases
por: Uppal, Sheetal, et al.
Publicado: (2020)

Stable Intronic Sequences and Exon Skipping Events in the Human RPE65 Gene: Analysis of Expression in Retinal Pigment Epithelium Cells and Cell Culture Models
por: Postnikova, Olga, et al.
Publicado: (2019)

The stability of envelope-pseudotyped lentiviral vectors
por: Dautzenberg, Iris J. C., et al.
Publicado: (2020)

Volatile Evolution of Long Non-Coding RNA Repertoire in Retinal Pigment Epithelium: Insights from Comparison of Bovine and Human RNA Expression Profiles
por: Postnikova, Olga A., et al.
Publicado: (2019)

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays
por: Crawford, Katharine H. D., et al.
Publicado: (2020)

Pseudotyping Lentiviral Vectors: When the Clothes Make the Virus
por: Duvergé, Alexis, et al.
Publicado: (2020)

The dual roles of RPE65 S-palmitoylation in membrane association and visual cycle function
por: Uppal, Sheetal, et al.
Publicado: (2019)

Improved GaLV-TR Glycoproteins to Pseudotype Lentiviral Vectors: Impact of Viral Protease Activity in the Production of LV Pseudotypes
por: Tomás, Hélio A., et al.
Publicado: (2019)

Pseudotyped Lentiviral Vectors for Retrograde Gene Delivery into Target Brain Regions
por: Kobayashi, Kenta, et al.
Publicado: (2017)

Pseudotyped lentiviral vectors: Ready for translation into targeted cancer gene therapy?
por: Deng, Longfei, et al.
Publicado: (2022)

Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
por: Poliakov, Eugenia, et al.
Publicado: (2017)

Low-Density Lipoprotein Receptor (LDLR) Family Orchestrates Cholesterol Homeostasis
por: Go, Gwang-woong, et al.
Publicado: (2012)

Characterization of low density lipoprotein receptor (LDLR) gene mutations in Albania
por: Diakou, Maria, et al.
Publicado: (2010)

Cleavage of Hemagglutinin-Bearing Lentiviral Pseudotypes and Their Use in the Study of Influenza Virus Persistence
por: Sawoo, Olivier, et al.
Publicado: (2014)

Xanthophylls Modulate Palmitoylation of Mammalian β-Carotene Oxygenase 2
por: Uppal, Sheetal, et al.
Publicado: (2021)

A robust lentiviral pseudotype neutralisation assay for in-field serosurveillance of rabies and lyssaviruses in Africa
por: Wright, Edward, et al.
Publicado: (2009)

Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison
por: Wright, Edward, et al.
Publicado: (2008)

Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing
por: Izumida, Mai, et al.
Publicado: (2020)

More Than Just Gene Therapy Vectors: Lentiviral Vector Pseudotypes for Serological Investigation
por: Toon, Kamilla, et al.
Publicado: (2021)

Novel Tools for Comprehensive Functional Analysis of LDLR (Low-Density Lipoprotein Receptor) Variants
por: Jasiecki, Jacek, et al.
Publicado: (2023)

Characterisation of SARS-CoV-2 Lentiviral Pseudotypes and Correlation between Pseudotype-Based Neutralisation Assays and Live Virus-Based Micro Neutralisation Assays
por: Hyseni, Inesa, et al.
Publicado: (2020)

Generalized Portrait of Cancer Metabolic Pathways Inferred from a List of Genes Overexpressed in Cancer
por: Poliakov, Eugenia, et al.
Publicado: (2014)

An inducible amphipathic α-helix mediates subcellular targeting and membrane binding of RPE65
por: Uppal, Sheetal, et al.
Publicado: (2022)

Chikungunya Virus Glycoproteins Pseudotype with Lentiviral Vectors and Reveal a Broad Spectrum of Cellular Tropism
por: Hu, Dakang, et al.
Publicado: (2014)

Analysis of the low density lipoprotein receptor gene (LDLR) mutation spectrum in Russian familial hypercholesterolemia
por: Vasilyev, V.B., et al.
Publicado: (2022)

Impairment of translation in neurons as a putative causative factor for autism
por: Poliakov, Eugenia, et al.
Publicado: (2014)

Optimized Pseudotyping Conditions for the SARS-COV-2 Spike Glycoprotein
por: Johnson, Marc C., et al.
Publicado: (2020)

Effective in vivo and ex vivo gene transfer to intestinal mucosa by VSV-G-pseudotyped lentiviral vectors
por: Matsumoto, Hiroshi, et al.
Publicado: (2010)

Induction of Broadly Neutralising HCV Antibodies in Mice by Integration-Deficient Lentiviral Vector-Based Pseudotyped Particles
por: Deng, Yao, et al.
Publicado: (2013)

Highly Efficient Retrograde Gene Transfer into Motor Neurons by a Lentiviral Vector Pseudotyped with Fusion Glycoprotein
por: Hirano, Miyabi, et al.
Publicado: (2013)

Mass spectrometry analysis reveals differences in the host cell protein species found in pseudotyped lentiviral vectors
por: Johnson, Sabine, et al.
Publicado: (2018)

The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
por: Farzaneh, Mostafa, et al.
Publicado: (2019)

Vectofusin-1 Promotes RD114-TR-Pseudotyped Lentiviral Vector Transduction of Human HSPCs and T Lymphocytes
por: Piovan, Claudia, et al.
Publicado: (2017)

Lentiviral Vector Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy
por: Gutierrez-Guerrero, Alejandra, et al.
Publicado: (2020)

Production of CRISPRi-engineered primary human mammary epithelial cells with baboon envelope pseudotyped lentiviral vectors
por: Pastor, Sonia, et al.
Publicado: (2023)

Meta-Analysis of Low Density Lipoprotein Receptor (LDLR) rs2228671 Polymorphism and Coronary Heart Disease
por: Ye, Huadan, et al.
Publicado: (2014)

Functional Analysis of LDLR (Low-Density Lipoprotein Receptor) Variants in Patient Lymphocytes to Assess the Effect of Evinacumab in Homozygous Familial Hypercholesterolemia Patients With a Spectrum of LDLR Activity
por: Banerjee, Poulabi, et al.
Publicado: (2019)

Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors
por: Hwang, Bum-Yeol, et al.
Publicado: (2013)

A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
por: Mournetas, Virginie, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_9mcpmjg87ttqv4p2o4el5f4153