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Structure, dynamics and transferability of the metal-dependent polyhistidine tetramerization motif TetrHis for single-chain Fv antibodies

The polyhistidine (6XHis) motif is one of the most ubiquitous protein purification tags. The 6XHis motif enables the binding of tagged proteins to various metals, which can be advantageously used for purification with immobilized metal affinity chromatography. Despite its popularity, protein structu...

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Detalles Bibliográficos
Autores principales: Healey, Robert D., Couillaud, Louise, Hoh, François, Mouhand, Assia, Fouillen, Aurelien, Couvineau, Pierre, Granier, Sébastien, Leyrat, Cédric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382482/
https://www.ncbi.nlm.nih.gov/pubmed/37507458
http://dx.doi.org/10.1038/s42004-023-00962-x