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Cell-surface biotinylation of GABA(A) receptors in mouse hippocampal slices after sevoflurane anesthesia

Here, we present a protocol for studying the cell-surface proteins in hippocampal slices after in vivo administration of sevoflurane, an inhaled general anesthetic drug, to mice. We describe steps for anesthetic delivery, hippocampal slice preparation, and cell-surface biotinylation. We then detail...

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Detalles Bibliográficos
Autores principales: Ariza, Anthony, Naeini, Setareh Malekian, Khodaei, Shahin, Ba, Joycelyn, Wang, Dian-Shi, Orser, Beverley Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382930/
https://www.ncbi.nlm.nih.gov/pubmed/37480561
http://dx.doi.org/10.1016/j.xpro.2023.102450