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Cell-surface biotinylation of GABA(A) receptors in mouse hippocampal slices after sevoflurane anesthesia
Here, we present a protocol for studying the cell-surface proteins in hippocampal slices after in vivo administration of sevoflurane, an inhaled general anesthetic drug, to mice. We describe steps for anesthetic delivery, hippocampal slice preparation, and cell-surface biotinylation. We then detail...
Autores principales: | Ariza, Anthony, Naeini, Setareh Malekian, Khodaei, Shahin, Ba, Joycelyn, Wang, Dian-Shi, Orser, Beverley Anne |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382930/ https://www.ncbi.nlm.nih.gov/pubmed/37480561 http://dx.doi.org/10.1016/j.xpro.2023.102450 |
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