Protocol for generating reproducible miniaturized controlled midbrain organoids

Here, we present a protocol for generating miniaturized controlled midbrain organoids (MiCOs) of reproducible size and cellular composition, without a necrotic center. We describe steps for maintaining and passaging human pluripotent stem cells, generating MiCOs using AggreWell(TM)400, and maintaini...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Muwan, Niclis, Jonathan Christos, Denham, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382973/
https://www.ncbi.nlm.nih.gov/pubmed/37481727
http://dx.doi.org/10.1016/j.xpro.2023.102451

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382973/
https://www.ncbi.nlm.nih.gov/pubmed/37481727
http://dx.doi.org/10.1016/j.xpro.2023.102451

Ejemplares similares