Cargando…

Protocol for generating reproducible miniaturized controlled midbrain organoids

Here, we present a protocol for generating miniaturized controlled midbrain organoids (MiCOs) of reproducible size and cellular composition, without a necrotic center. We describe steps for maintaining and passaging human pluripotent stem cells, generating MiCOs using AggreWell(TM)400, and maintaini...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chen, Muwan, Niclis, Jonathan Christos, Denham, Mark
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382973/ https://www.ncbi.nlm.nih.gov/pubmed/37481727 http://dx.doi.org/10.1016/j.xpro.2023.102451

Ejemplares similares

A robust protocol for the generation of human midbrain organoids
por: Zagare, Alise, et al.
Publicado: (2021)

High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells
por: Sarrafha, Lily, et al.
Publicado: (2021)

An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
por: Gantner, Carlos W., et al.
Publicado: (2020)

Protocol for generation of lung adenocarcinoma organoids from clinical samples
por: Li, Zhichao, et al.
Publicado: (2020)

Generation and characterization of NGLY1 patient-derived midbrain organoids
por: Abbott, Joshua, et al.
Publicado: (2023)

Protocol for generating embedding-free brain organoids enriched with oligodendrocytes
por: Al-mhanawi, Bahaa, et al.
Publicado: (2023)

Protocol to generate large human intestinal organoids using a rotating bioreactor
por: Takahashi, Junichi, et al.
Publicado: (2023)

Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
por: Ryosaka, Makoto, et al.
Publicado: (2022)

Generation of Pancreatic Organoid-Derived Isografts
por: D’Agosto, Sabrina, et al.
Publicado: (2020)

Equine Oviductal Organoid Generation and Cryopreservation
por: Thompson, Riley E., et al.
Publicado: (2022)

A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
por: Nishimura, Kaneyasu, et al.
Publicado: (2023)

Modeling Parkinson’s disease in midbrain-like organoids
por: Smits, Lisa M., et al.
Publicado: (2019)

Generation and functional characterization of murine mammary organoids
por: Yip, Hon Yan Kelvin, et al.
Publicado: (2021)

Protocol for tissue clearing and 3D analysis of dopamine neurons in the developing mouse midbrain
por: Adolfs, Youri, et al.
Publicado: (2021)

Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
por: Ford, Matthew J., et al.
Publicado: (2022)

Optimized Transgene Delivery Using Third‐Generation Lentiviruses
por: Gill, Katherine P., et al.
Publicado: (2020)

Generation of human embryonic stem cell-derived lung organoids
por: Han, Le, et al.
Publicado: (2022)

Protocol to profile the bioenergetics of organoids using Seahorse
por: Ludikhuize, Marlies C., et al.
Publicado: (2021)

Generating Patient-Derived Gliomas within Cerebral Organoids
por: Linkous, Amanda, et al.
Publicado: (2020)

Generation of mixed murine organoids to model cellular interactions
por: Krotenberg Garcia, Ana, et al.
Publicado: (2021)

HIPRO: A High-Efficiency, Hypoxia-Induced Protocol for Generation of Photoreceptors in Retinal Organoids from Mouse Pluripotent Stem Cells
por: Chen, Holly Y., et al.
Publicado: (2020)

Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury
por: Ramirez, Santiago, et al.
Publicado: (2021)

An efficient method to generate kidney organoids at the air-liquid interface
por: Gupta, Ashwani Kumar, et al.
Publicado: (2021)

Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell-derived cerebral organoids
por: Feng, Yilin, et al.
Publicado: (2023)

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease
por: Smits, Lisa Maria, et al.
Publicado: (2020)

Generation and validation of APOE knockout human iPSC-derived cerebral organoids
por: Martens, Yuka A., et al.
Publicado: (2021)

Generation of vascularized human cardiac organoids for 3D in vitro modeling
por: Voges, Holly K., et al.
Publicado: (2023)

Generation, culture, and stimulation of small intestinal murine organoids in parasitology research
por: Campillo Poveda, Marta, et al.
Publicado: (2023)

Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells
por: Wörsdörfer, Philipp, et al.
Publicado: (2020)

Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development
por: Xiang, Yangfei, et al.
Publicado: (2020)

Protocol for establishing a coculture with fibroblasts and colorectal cancer organoids
por: Wallisch, Svenja, et al.
Publicado: (2023)

Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids
por: Ober, Kimberley, et al.
Publicado: (2023)

Protocol for establishing organoids from human ovarian cancer biopsies
por: Maenhoudt, Nina, et al.
Publicado: (2021)

Protocols to culture and harvest hepatic tumor organoids for metabolic assays
por: Tong, Man, et al.
Publicado: (2022)

Midbrain organoids with an SNCA gene triplication model key features of synucleinopathy
por: Mohamed, Nguyen-Vi, et al.
Publicado: (2021)

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
por: Huber, Anne, et al.
Publicado: (2023)

Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
por: Kim, Hyosung, et al.
Publicado: (2021)

Generation of cell-type-specific proteomes of neurodevelopment from human cerebral organoids
por: Melliou, Sofia, et al.
Publicado: (2022)

Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
por: Ali, Ayesha, et al.
Publicado: (2020)

Intestinal organoid co-culture protocol to study cell competition in vitro
por: van Neerven, Sanne M., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_e8ue41kerh8ide0q2fgnstbbim