Cargando…

Development of an Alcohol Dilution–Lyophilization Method for the Preparation of mRNA-LNPs with Improved Storage Stability

The lipid nanoparticle (LNP) is one of the promising nanotechnologies for the delivery of RNA molecules, such as small interfering RNA (siRNA) and messenger RNA (mRNA). A series of LNPs that contain an mRNA encoding the antigen protein of SARS-CoV-2 were already approved as RNA vaccines against this...

Descripción completa

Detalles Bibliográficos
Autores principales:	Shirane, Daiki, Tanaka, Hiroki, Sakurai, Yu, Taneichi, Sakura, Nakai, Yuta, Tange, Kota, Ishii, Itsuko, Akita, Hidetaka
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383539/ https://www.ncbi.nlm.nih.gov/pubmed/37514007 http://dx.doi.org/10.3390/pharmaceutics15071819

Ejemplares similares

Improvement of mRNA Delivery Efficiency to a T Cell Line by Modulating PEG-Lipid Content and Phospholipid Components of Lipid Nanoparticles
por: Tanaka, Hiroki, et al.
Publicado: (2021)

Lyophilization process optimization and molecular dynamics simulation of mRNA-LNPs for SARS-CoV-2 vaccine
por: Li, Mingyuan, et al.
Publicado: (2023)

Tolerogenic Lipid Nanoparticles for Delivering Self-Antigen mRNA for the Treatment of Experimental Autoimmune Encephalomyelitis
por: Gomi, Masaki, et al.
Publicado: (2023)

Delivery of Oligonucleotides Using a Self-Degradable Lipid-Like Material
por: Tanaka, Hiroki, et al.
Publicado: (2021)

Unmodified mRNA in LNPs constitutes a competitive technology for prophylactic vaccines
por: Lutz, Johannes, et al.
Publicado: (2017)

SV-AUC as a stability-indicating method for the characterization of mRNA-LNPs
por: Thaller, Anian, et al.
Publicado: (2023)

An Ionizable Lipid Material with a Vitamin E Scaffold as an mRNA Vaccine Platform for Efficient Cytotoxic T Cell Responses
por: Oyama, Ryotaro, et al.
Publicado: (2023)

pH-Responsive Lipid Nanoparticles Achieve Efficient mRNA Transfection in Brain Capillary Endothelial Cells
por: Sakurai, Yu, et al.
Publicado: (2022)

Biodistribution and Non-linear Gene Expression of mRNA LNPs Affected by Delivery Route and Particle Size
por: Di, Jiaxing, et al.
Publicado: (2022)

CAR-NK Cells Generated with mRNA-LNPs Kill Tumor Target Cells In Vitro and In Vivo
por: Golubovskaya, Vita, et al.
Publicado: (2023)

siRNA Functionalized Lipid Nanoparticles (LNPs) in Management of Diseases
por: Kalita, Tutu, et al.
Publicado: (2022)

The delivery of mRNA to colon inflammatory lesions by lipid-nano-particles containing environmentally-sensitive lipid-like materials with oleic acid scaffolds
por: Tanaka, Hiroki, et al.
Publicado: (2018)

Am80-Encapsulated Lipid Nanoparticles, Developed with the Aim of Achieving Alveolar Regeneration, Have an Improvement Effect on Pulmonary Emphysema
por: Akita, Tomomi, et al.
Publicado: (2022)

Intracellular Drug Delivery Process of Am80-Encapsulated Lipid Nanoparticles Aiming for Alveolar Regeneration
por: Akita, Tomomi, et al.
Publicado: (2023)

Studying how administration route and dose regulates antibody generation against LNPs for mRNA delivery with single-particle resolution
por: Münter, Rasmus, et al.
Publicado: (2023)

Highly efficient CD4+ T cell targeting and genetic recombination using engineered CD4+ cell-homing mRNA-LNPs
por: Tombácz, István, et al.
Publicado: (2021)

Effect of Cholesterol Content of Lipid Composition in mRNA-LNPs on the Protein Expression in the Injected Site and Liver After Local Administration in Mice
por: Kawaguchi, Maho, et al.
Publicado: (2023)

Improved lyophilization conditions for long-term storage of bacteriophages
por: Manohar, Prasanth, et al.
Publicado: (2019)

Development of a Lyophilization Process for Campylobacter Bacteriophage Storage and Transport
por: Liang, Lu, et al.
Publicado: (2020)

Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primates
por: Suzuki, Yuta, et al.
Publicado: (2022)

Effects of lyophilization and storage temperature on Wuchereria bancrofti antigen sensitivity and stability
por: Agbozo, Edem Y., et al.
Publicado: (2018)

Effects on Metabolism in Astrocytes Caused by cGAMP, Which Imitates the Initial Stage of Brain Metastasis
por: Okawa, Toya, et al.
Publicado: (2021)

Silencing of VEGFR2 by RGD-Modified Lipid Nanoparticles Enhanced the Efficacy of Anti-PD-1 Antibody by Accelerating Vascular Normalization and Infiltration of T Cells in Tumors
por: Cho, Riki, et al.
Publicado: (2020)

The effect of lyophilization and storage time on the survival rate and hydrolytic activity of Trichoderma strains
por: Grzegorczyk, Monika, et al.
Publicado: (2018)

The evaluation of novel oral vaccines based on self-amplifying RNA lipid nanparticles (saRNA LNPs), saRNA transfected Lactobacillus plantarum LNPs, and saRNA transfected Lactobacillus plantarum to neutralize SARS-CoV-2 variants alpha and delta
por: Keikha, Reza, et al.
Publicado: (2021)

Preparation and spectroscopic characterization of lyophilized Mo nitrogenase
por: Van Stappen, Casey, et al.
Publicado: (2020)

Development of Thermostable Lyophilized Sabin Inactivated Poliovirus Vaccine
por: Shin, Woo-Jin, et al.
Publicado: (2018)

Efficient mRNA Delivery with Lyophilized Human Serum Albumin-Based Nanobubbles
por: Kida, Hiroshi, et al.
Publicado: (2023)

Antimicrobial Effects of Basil, Summer Savory and Tarragon Lyophilized Extracts in Cold Storage Sausages
por: Macari, Artur, et al.
Publicado: (2021)

A Single Dose of Anti-HBsAg Antibody-Encoding mRNA-LNPs Suppressed HBsAg Expression: a Potential Cure of Chronic Hepatitis B Virus Infection
por: Chen, Binfan, et al.
Publicado: (2022)

Development of lyophilization cycle and effect of excipients on the stability of catalase during lyophilization
por: Lale, Shantanu V, et al.
Publicado: (2011)

DoE-derived continuous and robust process for manufacturing of pharmaceutical-grade wide-range LNPs for RNA-vaccine/drug delivery
por: Nag, Kakon, et al.
Publicado: (2022)

Established Liposome-Coated IMB16-4 Polymeric Nanoparticles (LNPs) for Increasing Cellular Uptake and Anti-Fibrotic Effects In Vitro
por: Niu, Xia, et al.
Publicado: (2022)

Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models
por: Wei, Tuo, et al.
Publicado: (2023)

In vivo genome editing using 244-cis LNPs and low-dose AAV achieves therapeutic threshold in hemophilia A mice
por: Han, Jeong Pil, et al.
Publicado: (2023)

Optimization of Sentinel Lymph Node Imaging Methodology Using Anionic Liposome and Hyaluronidase
por: Sakurai, Yu, et al.
Publicado: (2021)

Effects of Processing and Storage on Pediococcus pentosaceus SB83 in Vaginal Formulations: Lyophilized Powder and Tablets
por: Borges, Sandra, et al.
Publicado: (2013)

Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics
por: De, Anindita, et al.
Publicado: (2022)

ABE8e Corrects Pax6-Aniridic Variant in Humanized Mouse ESCs and via LNPs in Ex Vivo Cortical Neurons
por: Adair, Bethany A., et al.
Publicado: (2023)

Paramagnetic changes in cancer: DMBA-induced tumours studied in non-lyophilized and lyophilized tissues.
por: Gutierrez, P. L., et al.
Publicado: (1979)

Cannot write session to /tmp/vufind_sessions/sess_vk6vbs0k610ouh9hl2t8odt8ms