Cargando…
The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice
Kale (Brassica oleracea var. acephala), a food rich in bioactive phytochemicals, prevents diet-induced inflammation and gut dysbiosis. We hypothesized that the phytochemicals protect against the lipopolysaccharide (LPS)-induced acute inflammation which results from gut dysbiosis and loss of gut barr...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383939/ https://www.ncbi.nlm.nih.gov/pubmed/37513639 http://dx.doi.org/10.3390/nu15143222 |
_version_ | 1785081034602184704 |
---|---|
author | Raychaudhuri, Samnhita Shahinozzaman, Md Subedi, Ujjwol Fan, Si Ogedengbe, Opeyemi Obanda, Diana N. |
author_facet | Raychaudhuri, Samnhita Shahinozzaman, Md Subedi, Ujjwol Fan, Si Ogedengbe, Opeyemi Obanda, Diana N. |
author_sort | Raychaudhuri, Samnhita |
collection | PubMed |
description | Kale (Brassica oleracea var. acephala), a food rich in bioactive phytochemicals, prevents diet-induced inflammation and gut dysbiosis. We hypothesized that the phytochemicals protect against the lipopolysaccharide (LPS)-induced acute inflammation which results from gut dysbiosis and loss of gut barrier integrity. We designed this study to test the protective effects of the whole vegetable by feeding C57BL/6J mice a rodent high-fat diet supplemented with or without 4.5% kale (0.12 g per 30 g mouse) for 2 weeks before administering 3% dextran sulfate sodium (DSS) via drinking water. After one week, DSS increased the representation of proinflammatory LPS (P-LPS)-producing genera Enterobacter and Klebsiella in colon contents, reduced the representation of anti-inflammatory LPS (A-LPS)-producing taxa from Bacteroidales, reduced the expression of tight junction proteins, increased serum LPS binding protein, upregulated molecular and histopathological markers of inflammation in the colon and shortened the colons. Mice fed kale for 2 weeks before the DSS regime had a significantly reduced representation of Enterobacter and Klebsiella and instead had increased Bacteroidales and Gram-positive taxa and enhanced expression of tight junction proteins. Downstream positive effects of dietary kale were lack of granuloma in colon samples, no shortening of the colon and prevention of inflammation; the expression of F4/80, TLR4 and cytokines 1L-1b, IL-6, TNF-a and iNOS was not different from that of the control group. We conclude that through reducing the proliferation of P-LPS-producing bacteria and augmenting the integrity of the gut barrier, kale protects against DSS-induced inflammation. |
format | Online Article Text |
id | pubmed-10383939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103839392023-07-30 The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice Raychaudhuri, Samnhita Shahinozzaman, Md Subedi, Ujjwol Fan, Si Ogedengbe, Opeyemi Obanda, Diana N. Nutrients Article Kale (Brassica oleracea var. acephala), a food rich in bioactive phytochemicals, prevents diet-induced inflammation and gut dysbiosis. We hypothesized that the phytochemicals protect against the lipopolysaccharide (LPS)-induced acute inflammation which results from gut dysbiosis and loss of gut barrier integrity. We designed this study to test the protective effects of the whole vegetable by feeding C57BL/6J mice a rodent high-fat diet supplemented with or without 4.5% kale (0.12 g per 30 g mouse) for 2 weeks before administering 3% dextran sulfate sodium (DSS) via drinking water. After one week, DSS increased the representation of proinflammatory LPS (P-LPS)-producing genera Enterobacter and Klebsiella in colon contents, reduced the representation of anti-inflammatory LPS (A-LPS)-producing taxa from Bacteroidales, reduced the expression of tight junction proteins, increased serum LPS binding protein, upregulated molecular and histopathological markers of inflammation in the colon and shortened the colons. Mice fed kale for 2 weeks before the DSS regime had a significantly reduced representation of Enterobacter and Klebsiella and instead had increased Bacteroidales and Gram-positive taxa and enhanced expression of tight junction proteins. Downstream positive effects of dietary kale were lack of granuloma in colon samples, no shortening of the colon and prevention of inflammation; the expression of F4/80, TLR4 and cytokines 1L-1b, IL-6, TNF-a and iNOS was not different from that of the control group. We conclude that through reducing the proliferation of P-LPS-producing bacteria and augmenting the integrity of the gut barrier, kale protects against DSS-induced inflammation. MDPI 2023-07-20 /pmc/articles/PMC10383939/ /pubmed/37513639 http://dx.doi.org/10.3390/nu15143222 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Raychaudhuri, Samnhita Shahinozzaman, Md Subedi, Ujjwol Fan, Si Ogedengbe, Opeyemi Obanda, Diana N. The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice |
title | The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice |
title_full | The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice |
title_fullStr | The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice |
title_full_unstemmed | The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice |
title_short | The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice |
title_sort | vegetable ‘kale’ protects against dextran-sulfate-sodium-induced acute inflammation through moderating the ratio of proinflammatory and anti-inflammatory lps-producing bacterial taxa and augmenting the gut barrier in c57bl6 mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383939/ https://www.ncbi.nlm.nih.gov/pubmed/37513639 http://dx.doi.org/10.3390/nu15143222 |
work_keys_str_mv | AT raychaudhurisamnhita thevegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT shahinozzamanmd thevegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT subediujjwol thevegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT fansi thevegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT ogedengbeopeyemi thevegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT obandadianan thevegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT raychaudhurisamnhita vegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT shahinozzamanmd vegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT subediujjwol vegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT fansi vegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT ogedengbeopeyemi vegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice AT obandadianan vegetablekaleprotectsagainstdextransulfatesodiuminducedacuteinflammationthroughmoderatingtheratioofproinflammatoryandantiinflammatorylpsproducingbacterialtaxaandaugmentingthegutbarrierinc57bl6mice |