Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry

Phosphorodiamidate morpholinos (PMOs) are known as premier gene knockdown tools in developmental biology. PMOs are usually 25 nucleo-base-long morpholino subunits with a neutral phosphorodiamidate linkage. PMOs work via a steric blocking mechanism and are stable towards nucleases’ inside cells. PMOs...

Descripción completa

Detalles Bibliográficos
Autores principales: Paul, Sibasish, Caruthers, Marvin H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384400/
https://www.ncbi.nlm.nih.gov/pubmed/37513252
http://dx.doi.org/10.3390/molecules28145380
_version_ 1785081148033990656
author Paul, Sibasish
Caruthers, Marvin H.
author_facet Paul, Sibasish
Caruthers, Marvin H.
author_sort Paul, Sibasish
collection PubMed
description Phosphorodiamidate morpholinos (PMOs) are known as premier gene knockdown tools in developmental biology. PMOs are usually 25 nucleo-base-long morpholino subunits with a neutral phosphorodiamidate linkage. PMOs work via a steric blocking mechanism and are stable towards nucleases’ inside cells. PMOs are usually synthesized using phosphoramidate P(V) chemistry. In this review, we will discuss the synthesis of PMOs, phosphoroamidate morpholinos (MO), and thiophosphoramidate morpholinos (TMO).
format Online
Article
Text
id pubmed-10384400
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103844002023-07-30 Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry Paul, Sibasish Caruthers, Marvin H. Molecules Review Phosphorodiamidate morpholinos (PMOs) are known as premier gene knockdown tools in developmental biology. PMOs are usually 25 nucleo-base-long morpholino subunits with a neutral phosphorodiamidate linkage. PMOs work via a steric blocking mechanism and are stable towards nucleases’ inside cells. PMOs are usually synthesized using phosphoramidate P(V) chemistry. In this review, we will discuss the synthesis of PMOs, phosphoroamidate morpholinos (MO), and thiophosphoramidate morpholinos (TMO). MDPI 2023-07-13 /pmc/articles/PMC10384400/ /pubmed/37513252 http://dx.doi.org/10.3390/molecules28145380 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Paul, Sibasish
Caruthers, Marvin H.
Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry
title Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry
title_full Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry
title_fullStr Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry
title_full_unstemmed Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry
title_short Synthesis of Backbone-Modified Morpholino Oligonucleotides Using Phosphoramidite Chemistry
title_sort synthesis of backbone-modified morpholino oligonucleotides using phosphoramidite chemistry
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384400/
https://www.ncbi.nlm.nih.gov/pubmed/37513252
http://dx.doi.org/10.3390/molecules28145380
work_keys_str_mv AT paulsibasish synthesisofbackbonemodifiedmorpholinooligonucleotidesusingphosphoramiditechemistry
AT caruthersmarvinh synthesisofbackbonemodifiedmorpholinooligonucleotidesusingphosphoramiditechemistry

Ejemplares similares