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Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing

Here, we present a protocol for spatially annotated single-cell sequencing, a technique for spatially profiling intratumor heterogeneity with deep single-cell RNA sequencing and single-cell resolution. By combining live-cell imaging and photopatterned illumination, we describe steps to identify regi...

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Detalles Bibliográficos
Autores principales: Smit, Myrthe M., Feller, Kate J., You, Li, Chien, Miao-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384656/
https://www.ncbi.nlm.nih.gov/pubmed/37453069
http://dx.doi.org/10.1016/j.xpro.2023.102447