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Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing
Here, we present a protocol for spatially annotated single-cell sequencing, a technique for spatially profiling intratumor heterogeneity with deep single-cell RNA sequencing and single-cell resolution. By combining live-cell imaging and photopatterned illumination, we describe steps to identify regi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384656/ https://www.ncbi.nlm.nih.gov/pubmed/37453069 http://dx.doi.org/10.1016/j.xpro.2023.102447 |