Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells

Here we present a protocol to generate standardized cerebral organoids with hippocampal regional specification using morphogen WNT3a. We describe steps for isolating mouse embryonic (E14.5) neural stem cells from the brain subgranular zone, preparing organoids samples for immunofluorescence, calcium...

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Detalles Bibliográficos
Autores principales: Ciarpella, Francesca, Zamfir, Raluca Georgiana, Campanelli, Alessandra, Pedrotti, Giulia, Di Chio, Marzia, Bottani, Emanuela, Decimo, Ilaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384661/
https://www.ncbi.nlm.nih.gov/pubmed/37454299
http://dx.doi.org/10.1016/j.xpro.2023.102413

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384661/
https://www.ncbi.nlm.nih.gov/pubmed/37454299
http://dx.doi.org/10.1016/j.xpro.2023.102413

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