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Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
The formation of water-insoluble complexes between chitosan (ChS) and caffeoylquinic acid (CQ) derivatives present in artichoke (AE) and green coffee bean (GCBE) extracts was investigated by the equilibrium adsorption method. The UPLC/HPLC analysis revealed that the phenolic compounds accounted for...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385370/ https://www.ncbi.nlm.nih.gov/pubmed/37513230 http://dx.doi.org/10.3390/molecules28145356 |
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author | Rosliuk, Deimante Jakstas, Valdas Ivanauskas, Liudas Liudvinaviciute, Dovile Coma, Veronique Rutkaite, Ramune |
author_facet | Rosliuk, Deimante Jakstas, Valdas Ivanauskas, Liudas Liudvinaviciute, Dovile Coma, Veronique Rutkaite, Ramune |
author_sort | Rosliuk, Deimante |
collection | PubMed |
description | The formation of water-insoluble complexes between chitosan (ChS) and caffeoylquinic acid (CQ) derivatives present in artichoke (AE) and green coffee bean (GCBE) extracts was investigated by the equilibrium adsorption method. The UPLC/HPLC analysis revealed that the phenolic compounds accounted for 8.1% and 74.6% of AE and GCBE respectively, and CQ derivatives were the predominant compounds. According to the applied Langmuir adsorption model, anionic compounds present in natural extracts were adsorbed onto the active centers of ChS, i.e., primary amino groups. The driving forces of adsorption were electrostatic interactions between cationic groups of ChS and anionic compounds of natural extracts. Chromatographic analysis revealed that not only CQ derivatives, but also other phenolic compounds of natural extracts were attached to ChS. The release of adsorbed compounds into different media as well as the bioactive properties of complexes were also studied. With the immobilization of bioactives onto ChS, increased and prolonged ABTS(•+) radical scavenging activity and decreased antifungal activity against Fusarium graminearum and Botrytis cinerea were observed compared to those of ChS. The findings of the current study highlight that the adsorption approach could be used to successfully prepare water-insoluble complexes of ChS and components of natural extracts with prolonged antioxidant activity. |
format | Online Article Text |
id | pubmed-10385370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103853702023-07-30 Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts Rosliuk, Deimante Jakstas, Valdas Ivanauskas, Liudas Liudvinaviciute, Dovile Coma, Veronique Rutkaite, Ramune Molecules Article The formation of water-insoluble complexes between chitosan (ChS) and caffeoylquinic acid (CQ) derivatives present in artichoke (AE) and green coffee bean (GCBE) extracts was investigated by the equilibrium adsorption method. The UPLC/HPLC analysis revealed that the phenolic compounds accounted for 8.1% and 74.6% of AE and GCBE respectively, and CQ derivatives were the predominant compounds. According to the applied Langmuir adsorption model, anionic compounds present in natural extracts were adsorbed onto the active centers of ChS, i.e., primary amino groups. The driving forces of adsorption were electrostatic interactions between cationic groups of ChS and anionic compounds of natural extracts. Chromatographic analysis revealed that not only CQ derivatives, but also other phenolic compounds of natural extracts were attached to ChS. The release of adsorbed compounds into different media as well as the bioactive properties of complexes were also studied. With the immobilization of bioactives onto ChS, increased and prolonged ABTS(•+) radical scavenging activity and decreased antifungal activity against Fusarium graminearum and Botrytis cinerea were observed compared to those of ChS. The findings of the current study highlight that the adsorption approach could be used to successfully prepare water-insoluble complexes of ChS and components of natural extracts with prolonged antioxidant activity. MDPI 2023-07-12 /pmc/articles/PMC10385370/ /pubmed/37513230 http://dx.doi.org/10.3390/molecules28145356 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rosliuk, Deimante Jakstas, Valdas Ivanauskas, Liudas Liudvinaviciute, Dovile Coma, Veronique Rutkaite, Ramune Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts |
title | Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts |
title_full | Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts |
title_fullStr | Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts |
title_full_unstemmed | Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts |
title_short | Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts |
title_sort | investigation of bioactive complexes of chitosan and green coffee bean or artichoke extracts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385370/ https://www.ncbi.nlm.nih.gov/pubmed/37513230 http://dx.doi.org/10.3390/molecules28145356 |
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