Cargando…

Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts

The formation of water-insoluble complexes between chitosan (ChS) and caffeoylquinic acid (CQ) derivatives present in artichoke (AE) and green coffee bean (GCBE) extracts was investigated by the equilibrium adsorption method. The UPLC/HPLC analysis revealed that the phenolic compounds accounted for...

Descripción completa

Detalles Bibliográficos
Autores principales: Rosliuk, Deimante, Jakstas, Valdas, Ivanauskas, Liudas, Liudvinaviciute, Dovile, Coma, Veronique, Rutkaite, Ramune
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385370/
https://www.ncbi.nlm.nih.gov/pubmed/37513230
http://dx.doi.org/10.3390/molecules28145356
_version_ 1785081389553549312
author Rosliuk, Deimante
Jakstas, Valdas
Ivanauskas, Liudas
Liudvinaviciute, Dovile
Coma, Veronique
Rutkaite, Ramune
author_facet Rosliuk, Deimante
Jakstas, Valdas
Ivanauskas, Liudas
Liudvinaviciute, Dovile
Coma, Veronique
Rutkaite, Ramune
author_sort Rosliuk, Deimante
collection PubMed
description The formation of water-insoluble complexes between chitosan (ChS) and caffeoylquinic acid (CQ) derivatives present in artichoke (AE) and green coffee bean (GCBE) extracts was investigated by the equilibrium adsorption method. The UPLC/HPLC analysis revealed that the phenolic compounds accounted for 8.1% and 74.6% of AE and GCBE respectively, and CQ derivatives were the predominant compounds. According to the applied Langmuir adsorption model, anionic compounds present in natural extracts were adsorbed onto the active centers of ChS, i.e., primary amino groups. The driving forces of adsorption were electrostatic interactions between cationic groups of ChS and anionic compounds of natural extracts. Chromatographic analysis revealed that not only CQ derivatives, but also other phenolic compounds of natural extracts were attached to ChS. The release of adsorbed compounds into different media as well as the bioactive properties of complexes were also studied. With the immobilization of bioactives onto ChS, increased and prolonged ABTS(•+) radical scavenging activity and decreased antifungal activity against Fusarium graminearum and Botrytis cinerea were observed compared to those of ChS. The findings of the current study highlight that the adsorption approach could be used to successfully prepare water-insoluble complexes of ChS and components of natural extracts with prolonged antioxidant activity.
format Online
Article
Text
id pubmed-10385370
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103853702023-07-30 Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts Rosliuk, Deimante Jakstas, Valdas Ivanauskas, Liudas Liudvinaviciute, Dovile Coma, Veronique Rutkaite, Ramune Molecules Article The formation of water-insoluble complexes between chitosan (ChS) and caffeoylquinic acid (CQ) derivatives present in artichoke (AE) and green coffee bean (GCBE) extracts was investigated by the equilibrium adsorption method. The UPLC/HPLC analysis revealed that the phenolic compounds accounted for 8.1% and 74.6% of AE and GCBE respectively, and CQ derivatives were the predominant compounds. According to the applied Langmuir adsorption model, anionic compounds present in natural extracts were adsorbed onto the active centers of ChS, i.e., primary amino groups. The driving forces of adsorption were electrostatic interactions between cationic groups of ChS and anionic compounds of natural extracts. Chromatographic analysis revealed that not only CQ derivatives, but also other phenolic compounds of natural extracts were attached to ChS. The release of adsorbed compounds into different media as well as the bioactive properties of complexes were also studied. With the immobilization of bioactives onto ChS, increased and prolonged ABTS(•+) radical scavenging activity and decreased antifungal activity against Fusarium graminearum and Botrytis cinerea were observed compared to those of ChS. The findings of the current study highlight that the adsorption approach could be used to successfully prepare water-insoluble complexes of ChS and components of natural extracts with prolonged antioxidant activity. MDPI 2023-07-12 /pmc/articles/PMC10385370/ /pubmed/37513230 http://dx.doi.org/10.3390/molecules28145356 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosliuk, Deimante
Jakstas, Valdas
Ivanauskas, Liudas
Liudvinaviciute, Dovile
Coma, Veronique
Rutkaite, Ramune
Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
title Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
title_full Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
title_fullStr Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
title_full_unstemmed Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
title_short Investigation of Bioactive Complexes of Chitosan and Green Coffee Bean or Artichoke Extracts
title_sort investigation of bioactive complexes of chitosan and green coffee bean or artichoke extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385370/
https://www.ncbi.nlm.nih.gov/pubmed/37513230
http://dx.doi.org/10.3390/molecules28145356
work_keys_str_mv AT rosliukdeimante investigationofbioactivecomplexesofchitosanandgreencoffeebeanorartichokeextracts
AT jakstasvaldas investigationofbioactivecomplexesofchitosanandgreencoffeebeanorartichokeextracts
AT ivanauskasliudas investigationofbioactivecomplexesofchitosanandgreencoffeebeanorartichokeextracts
AT liudvinaviciutedovile investigationofbioactivecomplexesofchitosanandgreencoffeebeanorartichokeextracts
AT comaveronique investigationofbioactivecomplexesofchitosanandgreencoffeebeanorartichokeextracts
AT rutkaiteramune investigationofbioactivecomplexesofchitosanandgreencoffeebeanorartichokeextracts