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Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice

Liver fibrosis resulting from chronic liver damage is becoming one of the major threats to health worldwide. Active saponin constituents isolated from Gynostemma pentaphyllum were found to possess a protective effect in liver diseases. Here, we obtained a naturally abundant gypenoside, XLVI, and eva...

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Autores principales: Li, Han, Wang, Hanghang, Yang, Aiping, Xue, Mingzhen, Wang, Junyang, Lv, Qi, Liu, Jian, Hu, Lihong, Zhang, Yinan, Wang, Xiachang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386501/
https://www.ncbi.nlm.nih.gov/pubmed/37513321
http://dx.doi.org/10.3390/molecules28145448
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author Li, Han
Wang, Hanghang
Yang, Aiping
Xue, Mingzhen
Wang, Junyang
Lv, Qi
Liu, Jian
Hu, Lihong
Zhang, Yinan
Wang, Xiachang
author_facet Li, Han
Wang, Hanghang
Yang, Aiping
Xue, Mingzhen
Wang, Junyang
Lv, Qi
Liu, Jian
Hu, Lihong
Zhang, Yinan
Wang, Xiachang
author_sort Li, Han
collection PubMed
description Liver fibrosis resulting from chronic liver damage is becoming one of the major threats to health worldwide. Active saponin constituents isolated from Gynostemma pentaphyllum were found to possess a protective effect in liver diseases. Here, we obtained a naturally abundant gypenoside, XLVI, and evaluated its liver protection activity in both animal and cellular models. The results showed that it ameliorated acute and chronic liver injuries and lightened the process of fibrogenesis in vivo. XLVI can inhibit TGF-β-induced activation of hepatic stellate cells and ECM deposition in vitro. The underlying mechanism study verified that it upregulated the protein expression of protein phosphatase 2C alpha and strengthened the vitality of the phosphatase together with a PP2Cα agonist gypenoside NPLC0393. These results shed new light on the molecular mechanisms and the potential therapeutic function of the traditional herb Gynostemma pentaphyllum in the treatment of liver fibrosis.
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spelling pubmed-103865012023-07-30 Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice Li, Han Wang, Hanghang Yang, Aiping Xue, Mingzhen Wang, Junyang Lv, Qi Liu, Jian Hu, Lihong Zhang, Yinan Wang, Xiachang Molecules Article Liver fibrosis resulting from chronic liver damage is becoming one of the major threats to health worldwide. Active saponin constituents isolated from Gynostemma pentaphyllum were found to possess a protective effect in liver diseases. Here, we obtained a naturally abundant gypenoside, XLVI, and evaluated its liver protection activity in both animal and cellular models. The results showed that it ameliorated acute and chronic liver injuries and lightened the process of fibrogenesis in vivo. XLVI can inhibit TGF-β-induced activation of hepatic stellate cells and ECM deposition in vitro. The underlying mechanism study verified that it upregulated the protein expression of protein phosphatase 2C alpha and strengthened the vitality of the phosphatase together with a PP2Cα agonist gypenoside NPLC0393. These results shed new light on the molecular mechanisms and the potential therapeutic function of the traditional herb Gynostemma pentaphyllum in the treatment of liver fibrosis. MDPI 2023-07-17 /pmc/articles/PMC10386501/ /pubmed/37513321 http://dx.doi.org/10.3390/molecules28145448 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Han
Wang, Hanghang
Yang, Aiping
Xue, Mingzhen
Wang, Junyang
Lv, Qi
Liu, Jian
Hu, Lihong
Zhang, Yinan
Wang, Xiachang
Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
title Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
title_full Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
title_fullStr Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
title_full_unstemmed Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
title_short Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
title_sort gypenosides synergistically reduce the extracellular matrix of hepatic stellate cells and ameliorate hepatic fibrosis in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386501/
https://www.ncbi.nlm.nih.gov/pubmed/37513321
http://dx.doi.org/10.3390/molecules28145448
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