Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector

A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline. The cloning efficiency is facilitated by a dual...

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Detalles Bibliográficos
Autores principales: Zare-Mehrjerdi, Omid, Trader, Gracie, Kirik, Viktor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2023
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388215/
https://www.ncbi.nlm.nih.gov/pubmed/37424091
http://dx.doi.org/10.2144/btn-2023-0001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388215/
https://www.ncbi.nlm.nih.gov/pubmed/37424091
http://dx.doi.org/10.2144/btn-2023-0001

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