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Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector
A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline. The cloning efficiency is facilitated by a dual...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Future Science Ltd
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388215/ https://www.ncbi.nlm.nih.gov/pubmed/37424091 http://dx.doi.org/10.2144/btn-2023-0001 |