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Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA

BACKGROUND: Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting Leptospira spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of Bst DNA polymerase. OBJECTIVE: To overcome the drawback of cold storage requirement for L...

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Detalles Bibliográficos
Autores principales: Lee, Pui-Yuei, Wong, Yien-Ping, Othman, Shuhaidah, Chee, Hui-Yee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388737/
https://www.ncbi.nlm.nih.gov/pubmed/37551329
http://dx.doi.org/10.2478/abm-2021-0023