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Low-cooling-rate freezing in biomolecular cryo-electron microscopy for recovery of initial frames
When biological samples are first exposed to electrons in cryo-electron microcopy (cryo-EM), proteins exhibit a rapid ‘burst’ phase of beam-induced motion that cannot be corrected with software. This lowers the quality of the initial frames, which are the least damaged by the electrons. Hence, they...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cambridge University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10392635/ https://www.ncbi.nlm.nih.gov/pubmed/37529673 http://dx.doi.org/10.1017/qrd.2021.8 |