Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis
Microglial activation during neuroinflammation is crucial for coordinating the immune response against neuronal tissue, and the initial response of microglia determines the severity of neuro-inflammatory diseases. The CD83 molecule has been recently shown to modulate the activation status of dendrit...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10394088/ https://www.ncbi.nlm.nih.gov/pubmed/37528070 http://dx.doi.org/10.1038/s41467-023-40370-2 |
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author | Sinner, Pia Peckert-Maier, Katrin Mohammadian, Hashem Kuhnt, Christine Draßner, Christina Panagiotakopoulou, Vasiliki Rauber, Simon Linnerbauer, Mathias Haimon, Zhana Royzman, Dmytro Kronenberg-Versteeg, Deborah Ramming, Andreas Steinkasserer, Alexander Wild, Andreas B. |
author_facet | Sinner, Pia Peckert-Maier, Katrin Mohammadian, Hashem Kuhnt, Christine Draßner, Christina Panagiotakopoulou, Vasiliki Rauber, Simon Linnerbauer, Mathias Haimon, Zhana Royzman, Dmytro Kronenberg-Versteeg, Deborah Ramming, Andreas Steinkasserer, Alexander Wild, Andreas B. |
author_sort | Sinner, Pia |
collection | PubMed |
description | Microglial activation during neuroinflammation is crucial for coordinating the immune response against neuronal tissue, and the initial response of microglia determines the severity of neuro-inflammatory diseases. The CD83 molecule has been recently shown to modulate the activation status of dendritic cells and macrophages. Although the expression of CD83 is associated with early microglia activation in various disease settings, its functional relevance for microglial biology has been elusive. Here, we describe a thorough assessment of CD83 regulation in microglia and show that CD83 expression in murine microglia is not only associated with cellular activation but also with pro-resolving functions. Using single-cell RNA-sequencing, we reveal that conditional deletion of CD83 results in an over-activated state during neuroinflammation in the experimental autoimmune encephalomyelitis model. Subsequently, CD83-deficient microglia recruit more pathogenic immune cells to the central nervous system, deteriorating resolving mechanisms and exacerbating the disease. Thus, CD83 in murine microglia orchestrates cellular activation and, consequently, also the resolution of neuroinflammation. |
format | Online Article Text |
id | pubmed-10394088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103940882023-08-03 Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis Sinner, Pia Peckert-Maier, Katrin Mohammadian, Hashem Kuhnt, Christine Draßner, Christina Panagiotakopoulou, Vasiliki Rauber, Simon Linnerbauer, Mathias Haimon, Zhana Royzman, Dmytro Kronenberg-Versteeg, Deborah Ramming, Andreas Steinkasserer, Alexander Wild, Andreas B. Nat Commun Article Microglial activation during neuroinflammation is crucial for coordinating the immune response against neuronal tissue, and the initial response of microglia determines the severity of neuro-inflammatory diseases. The CD83 molecule has been recently shown to modulate the activation status of dendritic cells and macrophages. Although the expression of CD83 is associated with early microglia activation in various disease settings, its functional relevance for microglial biology has been elusive. Here, we describe a thorough assessment of CD83 regulation in microglia and show that CD83 expression in murine microglia is not only associated with cellular activation but also with pro-resolving functions. Using single-cell RNA-sequencing, we reveal that conditional deletion of CD83 results in an over-activated state during neuroinflammation in the experimental autoimmune encephalomyelitis model. Subsequently, CD83-deficient microglia recruit more pathogenic immune cells to the central nervous system, deteriorating resolving mechanisms and exacerbating the disease. Thus, CD83 in murine microglia orchestrates cellular activation and, consequently, also the resolution of neuroinflammation. Nature Publishing Group UK 2023-08-01 /pmc/articles/PMC10394088/ /pubmed/37528070 http://dx.doi.org/10.1038/s41467-023-40370-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sinner, Pia Peckert-Maier, Katrin Mohammadian, Hashem Kuhnt, Christine Draßner, Christina Panagiotakopoulou, Vasiliki Rauber, Simon Linnerbauer, Mathias Haimon, Zhana Royzman, Dmytro Kronenberg-Versteeg, Deborah Ramming, Andreas Steinkasserer, Alexander Wild, Andreas B. Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
title | Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
title_full | Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
title_fullStr | Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
title_full_unstemmed | Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
title_short | Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
title_sort | microglial expression of cd83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10394088/ https://www.ncbi.nlm.nih.gov/pubmed/37528070 http://dx.doi.org/10.1038/s41467-023-40370-2 |
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