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A multiplexed bacterial two-hybrid for rapid characterization of protein–protein interactions and iterative protein design

Protein-protein interactions (PPIs) are crucial for biological functions and have applications ranging from drug design to synthetic cell circuits. Coiled-coils have been used as a model to study the sequence determinants of specificity. However, building well-behaved sets of orthogonal pairs of coi...

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Detalles Bibliográficos
Autores principales: Boldridge, W. Clifford, Ljubetič, Ajasja, Kim, Hwangbeom, Lubock, Nathan, Szilágyi, Dániel, Lee, Jonathan, Brodnik, Andrej, Jerala, Roman, Kosuri, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10397247/
https://www.ncbi.nlm.nih.gov/pubmed/37532706
http://dx.doi.org/10.1038/s41467-023-38697-x