Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina

Investigation of nuclear lamina architecture relies on superresolved microscopy. However, epitope accessibility, labeling density, and detection precision of individual molecules pose challenges within the molecularly crowded nucleus. We developed iterative indirect immunofluorescence (IT-IF) staini...

Descripción completa

Detalles Bibliográficos
Autores principales: Mäntylä, Elina, Montonen, Toni, Azzari, Lucio, Mattola, Salla, Hannula, Markus, Vihinen-Ranta, Maija, Hyttinen, Jari, Vippola, Minnamari, Foi, Alessandro, Nymark, Soile, Ihalainen, Teemu O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2023
Materias:
Special Issue on Quantitative Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10398900/
https://www.ncbi.nlm.nih.gov/pubmed/37342871
http://dx.doi.org/10.1091/mbc.E22-09-0448

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10398900/
https://www.ncbi.nlm.nih.gov/pubmed/37342871
http://dx.doi.org/10.1091/mbc.E22-09-0448

Ejemplares similares