p-Phenylene Diisothiocyanate-Based Covalent Immobilization of β-d-Galactosidase and Determination of Enzyme Activity by Cleavage of X-Gal and ONPG on Solid Support

[Image: see text] Herein, we present the immobilization of a technical grade β-d-galactosidase on amino-functionalized microtiter plates. Afterward, we transferred the results to a resin-based approach. For the covalent binding of the enzyme, an amino-functionalized microtiter plate was prefunctiona...

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Detalles Bibliográficos
Autores principales: Dayi, Defne I., Eschenhagen, Ursula, Seidinger, Henrike, Schneider, Holger, Schmidt, Magnus S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399185/
https://www.ncbi.nlm.nih.gov/pubmed/37546597
http://dx.doi.org/10.1021/acsomega.3c03279

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399185/
https://www.ncbi.nlm.nih.gov/pubmed/37546597
http://dx.doi.org/10.1021/acsomega.3c03279

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