Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

Targeted mutagenesis of a promoter or gene is essential for attaining new functions in microbial and protein engineering efforts. In the burgeoning field of synthetic biology, heterologous genes are expressed in new host organisms. Similarly, natural or designed proteins are mutagenized at targeted...

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Detalles Bibliográficos
Autores principales: Huttanus, Herbert M., Triola, Ellin-Kristina H., Velasquez-Guzman, Jeanette C., Shin, Sang-Min, Granja-Travez, Rommel S., Singh, Anmoldeep, Dale, Taraka, Jha, Ramesh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400447/
https://www.ncbi.nlm.nih.gov/pubmed/37545889
http://dx.doi.org/10.3389/fbioe.2023.1202388

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400447/
https://www.ncbi.nlm.nih.gov/pubmed/37545889
http://dx.doi.org/10.3389/fbioe.2023.1202388

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