16S rRNA gene primer choice impacts off-target amplification in human gastrointestinal tract biopsies and microbiome profiling

16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification of human DNA, current protocols are inadequat...

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Detalles Bibliográficos
Autores principales: Deissová, Tereza, Zapletalová, Martina, Kunovský, Lumír, Kroupa, Radek, Grolich, Tomáš, Kala, Zdeněk, Bořilová Linhartová, Petra, Lochman, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400661/
https://www.ncbi.nlm.nih.gov/pubmed/37537336
http://dx.doi.org/10.1038/s41598-023-39575-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400661/
https://www.ncbi.nlm.nih.gov/pubmed/37537336
http://dx.doi.org/10.1038/s41598-023-39575-8

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