Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons

Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA...

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Detalles Bibliográficos
Autores principales: Sheta, Razan, Teixeira, Maxime, Idi, Walid, Oueslati, Abid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400954/
https://www.ncbi.nlm.nih.gov/pubmed/37515763
http://dx.doi.org/10.1016/j.xpro.2023.102486
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author Sheta, Razan
Teixeira, Maxime
Idi, Walid
Oueslati, Abid
author_facet Sheta, Razan
Teixeira, Maxime
Idi, Walid
Oueslati, Abid
author_sort Sheta, Razan
collection PubMed
description Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks. For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).(1)
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spelling pubmed-104009542023-08-05 Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons Sheta, Razan Teixeira, Maxime Idi, Walid Oueslati, Abid STAR Protoc Protocol Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks. For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).(1) Elsevier 2023-07-28 /pmc/articles/PMC10400954/ /pubmed/37515763 http://dx.doi.org/10.1016/j.xpro.2023.102486 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sheta, Razan
Teixeira, Maxime
Idi, Walid
Oueslati, Abid
Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
title Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
title_full Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
title_fullStr Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
title_full_unstemmed Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
title_short Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
title_sort optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400954/
https://www.ncbi.nlm.nih.gov/pubmed/37515763
http://dx.doi.org/10.1016/j.xpro.2023.102486
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