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Protocol for analyzing antibody responses to glycoprotein antigens using electron-microscopy-based polyclonal epitope mapping

Electron microscopy-based polyclonal epitope mapping (EMPEM) can delineate epitope specificities of serum antibodies to a given antigen following vaccination or infection. Here, we present a protocol for the EMPEM method for rapid high-throughput assessment of antibody responses to glycoprotein anti...

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Detalles Bibliográficos
Autores principales: Turner, Hannah L., Jackson, Abigail M., Richey, Sara T., Sewall, Leigh M., Antanasijevic, Aleksandar, Hangartner, Lars, Ward, Andrew B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400963/
https://www.ncbi.nlm.nih.gov/pubmed/37516970
http://dx.doi.org/10.1016/j.xpro.2023.102476