Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET

Two-hybrid Förster resonance energy transfer (FRET) provides proximity, affinity, and stoichiometry information in binding interactions. We present an image-based approach that surpasses traditional two-hybrid FRET assays in precision and robustness. We outline instrument setup and image acquisition...

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Detalles Bibliográficos
Autores principales: Feldmann, Colin, Schänzler, Michael, Ben-Johny, Manu, Wahl-Schott, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400964/
https://www.ncbi.nlm.nih.gov/pubmed/37516972
http://dx.doi.org/10.1016/j.xpro.2023.102459

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400964/
https://www.ncbi.nlm.nih.gov/pubmed/37516972
http://dx.doi.org/10.1016/j.xpro.2023.102459

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