Efficient tagging of endogenous proteins in human cell lines for structural studies by single-particle cryo-EM

CRISPR/Cas9-based genome engineering has revolutionized our ability to manipulate biological systems, particularly in higher organisms. Here, we designed a set of homology-directed repair donor templates that enable efficient tagging of endogenous proteins with affinity tags by transient transfectio...

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Detalles Bibliográficos
Autores principales: Choi, Wooyoung, Wu, Hao, Yserentant, Klaus, Huang, Bo, Cheng, Yifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401002/
https://www.ncbi.nlm.nih.gov/pubmed/37487103
http://dx.doi.org/10.1073/pnas.2302471120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401002/
https://www.ncbi.nlm.nih.gov/pubmed/37487103
http://dx.doi.org/10.1073/pnas.2302471120

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