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Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria

BACKGROUND: Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA ex...

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Detalles Bibliográficos
Autores principales: Gand, Mathieu, Bloemen, Bram, Vanneste, Kevin, Roosens, Nancy H. C., De Keersmaecker, Sigrid C. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401787/
https://www.ncbi.nlm.nih.gov/pubmed/37537550
http://dx.doi.org/10.1186/s12864-023-09537-5