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A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis

ATP, an important cofactor, is involved in many biocatalytic reactions that require energy. Polyphosphate kinases (PPK) can provide energy for ATP-consuming reactions due to their cheap and readily available substrate polyphosphate. We determined the catalytic properties of PPK from different source...

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Detalles Bibliográficos
Autores principales: Gao, Hui, Li, Mengxuan, Wang, Qing, Liu, Tingting, Zhang, Xian, Yang, Taowei, Xu, Meijuan, Rao, Zhiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401862/
https://www.ncbi.nlm.nih.gov/pubmed/37537682
http://dx.doi.org/10.1186/s13068-023-02361-9