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Application of monolayer graphene to cryo-electron microscopy grids for high-resolution structure determination
In cryogenic electron microscopy (cryo-EM), purified macromolecules are typically applied to a grid bearing a holey carbon foil, blotted to remove excess liquid and rapidly frozen in a roughly 20–100 nm thick layer of vitreous ice that is suspended across roughly 1 μm-wide foil holes. The resulting...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402136/ https://www.ncbi.nlm.nih.gov/pubmed/37546934 http://dx.doi.org/10.1101/2023.07.28.550908 |